American College of Medical Genetics and Genomics

STANDARDS AND GUIDELINES FOR CLINICAL GENETICS LABORATORIES

2008 Edition, Revised 02/2007

 

(For a general overview of these Standards and Guidelines, including purpose and disclaimer, see Section A)

 

 

 

 

D

SHARED METHODOLOGIES

 

D1

Cell Culture

 

D1.1

Biosafety containment cabinets (Class IIA or IIB) must be used for all cell cultures and for the handling of viable tissues and/or fluids.

 

D1.2

Incubators

 

D1.2.1

Two incubators on separate electrical circuits (if no emergency backup power is available) are required for all amniotic fluid and chorionic villi cell cultures. These should have separate CO2 lines and filters, and should have emergency temperature alarms.

 

D1.2.2

Incubators must be cleaned regularly, and incubators must be monitored for 1) temperature each working day, 2) gas weekly, and 3) humidity, as needed. Maximum-minimum control thermometers are recommended. Appropriate operating ranges for equipment should be established and posted. Protocols should outline steps to be taken when readings are outside of appropriate ranges.